The Investigation of Some Phytochemical Compounds Found in Anchusa strigosa L. Grown Naturally in Iraq

Anchusa strigosa L.: Hardy annual biennial or perennial herb, with hairs especially on the leaves., flowers generally regular. Commonly named (Lisan Althour) in Iraq, from Boraginaceae family. The plant contains phenolic acids, flavonoids, alkaloids, sterols, and terpenoids. Whole part defatted n-hexane for 24 hours. material extracted using absolute methanol by Soxhlet apparatus hours, extract fractionated solvents of different polarity: petroleum ether- chloroform - ethylacetate- n-butanol respectively. fraction hydrolyzed 10% HCl 5 hours reflex to break down glycosidic linkage. Rosmarinic acid, caffeic genistein, silybin were isolated ethyl acetate preparative layer chromatography which identified high performance liquid HPLC, Fourier transforms infrared (FTIR) spectra, thin- TLC melting point. Since contain alkaloids so acid- base extraction performed crude resulting maceration parts (cold method) obtain alkaloid that then spectra thin-layer (TLC).
 aim this research was carry out a phytochemical study since no previous investigation work had been done species Iraq.